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7 cell cultures was decided using an ELISA kit in accordance spss spss manufacturer's instructions. RAW 264. 7 cells were incubated with LPS 0. 1 g/ml in spss presence of alternative concentrations of ACS extract for 24 hr. Absorbance was measured at 450 nm using spss help microplate reader and compared spss spss help regular curve spss examine spss pro inflammatory cytokine levels existing in spss supernatants. DPPH radical scavenging recreation of spss extracts was evaluated according spss spss method of Hatano and Kagawa . ACS extracts were added spss 100 l of DPPH solution spss spss help final awareness of 0. 2 mM, mixed gently, and allowed spss stand for 30 min. The absorbance of spss combination was measured at 517 nm with spss help microplate reader. Tocopherol was used as spss help constructive control average and DPPH radical scavenging activity was calculated using spss following equation:Antimicrobial undertaking was decided using spss agar disc diffusion method or broth micro dilution susceptibility assay. A.

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